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Chemical Biology BioNanotechnology Synthetic Biology Nanobiomaterial Science | ![]() |
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ELP
(elastin like protein) as scaffold materials and basis of ECM mimicking environments for eucaryotic cells Cellular adhesion and cell growth of hMSC (human mesenchymal stroma cells) cultures on ELP block-copolymer coated surfaces ![]() Comparison of cell adhesion and cell growth profiles of four different hMSC (human mesenchymal stroma cell) cultures on an ELP coated surface. hMSC of four donors were investigated: SC02_11, SC003, SC099, SC109 and incubated on seven different ELP polymer coatings as indicated: R20F20, mEGFP-E20F20, F20C1, (V2Y)F20, V20, E40-mEGFP, (V2Y)15 and uncoated well. A. Cell adhesion of four hMSC cultures on seven different ELP polymer coated surfaces. Cell adhesion was monitored by microscopy and determined after 4h by calculating the relative DNA amount of the adhered cells. B. Cell growth of four hMSC cultures on seven different ELP polymer coated surfaces. Cell growth was monitored by microscopy and determined after 72h of incubation by calculating the relative DNA amount of the adhered cells compared to the DNA amount after primary adhesion was completed (4h). Cellular adhesion and cell growth of fibroblast cells on ELP block-copolymer coated surfaces ![]() In the first column the protein-tectons used for the surface coating experiments on petri dishes were listed. The petri dishes were incubated with comparable amounts of different coating proteins. They were sterilized with 70% Ethanol and UV 250nm treatment and air dried. The second column show the results of cell adhesion experiments performed with NHDF (normal human dermal fibroblast) cells. Petri dishes were incubated with 7ml of culture medium for 1 hour and 1.2x106 cells/ml were released to each dish. Micrographs were taken after 4 days of incubation and percentage of coverage was determined manually. The third column show the results of cell adhesion experiments performed with L929 (mouse fibroblast) cells. Petri dishes were incubated with 7ml of culture medium for 1 hour and 0.8x106 cells/ml were released to each dish. Micrographs were taken after 4 days of incubation and percentage of coverage was determined manually. Biogenic Materials: Properties of elastine like protein (ELP) based amphiphilic block-copolymer ![]() Huber, M. C., Schreiber, A., Wild, W., Benz, K., Schiller, S. M. "Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid materials" Biomaterials 2014, 35, 8767-8779 |
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Revised April 2015 by M. C. Huber |