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Biohybrid Nanoarchitectures:
Protein Adaptor Based Nano-Object Assembly
(PABNOA)

Functional Expansion of the Cell:
De Novo Organells &
Cellular Modules and Nanoreactors

Biogenic Materials:
Elastine like Proteins
ECM mimicking matrix proteins

Sustainable Biopolymers &
Scaffolds in
Regenerative Medicine

Bioorthogonal Chemistry & Bioconjugation:
Unnatural Amino Acids
Glycolipid Library Tool Box

Modular Molecular Tecton Library
for Biogenic Materials
by One-Vector-Toolbox-Platform (OVTP)

Bioprocess & Enzyme engineering
Peptide Generator
for Biopharmaceuticals

Biomimetic Systems
Membranebiochemistry for
tethered bilayer lipid membranes (tBLM)




       










































ELP (elastin like protein) as scaffold materials and basis of ECM mimicking environments for eucaryotic cells


Cellular adhesion and cell growth of hMSC (human mesenchymal stroma cells) cultures on ELP block-copolymer coated surfaces

hMSC adhesion on ELPs
Comparison of cell adhesion and cell growth profiles of four different hMSC (human mesenchymal stroma cell) cultures on an ELP coated surface.
hMSC of four donors were investigated: SC02_11, SC003, SC099, SC109 and incubated on seven different ELP polymer coatings as indicated:
R20F20, mEGFP-E20F20, F20C1, (V2Y)F20, V20, E40-mEGFP, (V2Y)15 and uncoated well.
A. Cell adhesion of four hMSC cultures on seven different ELP polymer coated surfaces. Cell adhesion was monitored by microscopy and determined
after 4h by calculating the relative DNA amount of the adhered cells.
B.  Cell growth of four hMSC cultures on seven different ELP polymer coated surfaces. Cell growth was monitored by microscopy and determined after
72h of incubation by calculating the relative DNA amount of the adhered cells compared to the DNA amount after primary adhesion was completed (4h).





Cellular adhesion and cell growth of fibroblast cells on ELP block-copolymer
coated surfaces

fibroblast cell growth
In the first column the protein-tectons used for the surface coating experiments on petri dishes were listed.
The petri dishes were incubated with comparable amounts of different coating proteins. They were sterilized with
70% Ethanol and UV 250nm treatment and air dried.

The second column show the results of cell adhesion experiments performed with NHDF (normal human dermal fibroblast)
cells. Petri dishes were incubated with 7ml of culture medium for 1 hour and 1.2x106 cells/ml were released to each dish.
Micrographs were taken after 4 days of incubation and percentage of coverage was determined manually.

The third column show the results of cell adhesion experiments performed with L929 (mouse fibroblast) cells.
Petri dishes were incubated with 7ml of culture medium for 1 hour and 0.8x106 cells/ml were released to each dish.
Micrographs were taken after 4 days of incubation and percentage of coverage was determined manually.





Biogenic Materials: Properties of elastine like protein (ELP) based amphiphilic block-copolymer

amphi ELPs


 



Huber, M. C., Schreiber, A., Wild, W., Benz, K., Schiller, S. M. "Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid materials" Biomaterials 2014, 35, 8767-8779
                                                      



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Revised April 2015 by M. C. Huber